Pieter de jong, phd
An Overview
Scientists & Staff
Patents & Support
BacPac
Publications

Email
Phone: 510-450-7919

 

Overview

Bacterial Artificial Chromosome Resources
In 2000, Dr. Pieter de Jong relocated his vast collection of human and animal DNA clones to CHORI from Roswell Park Cancer Institute in Buffalo, New York. This collection is the major source of clone material for the Human Genome Project. Additionally, researchers worldwide use the collection to characterize genomes of many medically and economically important species of animals, protists, and bacteria.

Using molecular biology techniques, Dr. de Jong breaks genomic DNA into very large segments and inserts the individual segments into a bacterial vector for propagation in E. coli. These bacterial artificial chromosomes (BAC) are amplified and stored in microtiter plates to create recombinant DNA libraries. To date, Dr. de Jong and his group have accumulated approximately three million clones, which are stored in forty freezers located at CHORI.

The major resource for decoding of the human genome is de Jong’s current human library, which comes from one anonymous donor. Because this person’s specific genetic background is completely unknown, research can proceed without distractions related to the ethnicity or personal characteristics of the donor.
The human library is his largest, but he also has libraries for the mouse, Drosophila, dog, cat, cow, pig, silkworm, and several strains of rat. Libraries of single-cell organisms include Plasmodium several trypanosomes, and other non-disease protists and bacteria. Future projects include creating libraries for additional medically relevant species and agricultural animals, including the horse, turkey, tilapia, and catfish.

Our site “BACPAC.chori.org” is down for maintenance and will be down until September 12.

We regret the inconvenience this creates for our customers.  Nevertheless we continue to accept and ship ordered BAC clones and vectors.  

Please email requests for quotations and ordering instructions to bacpacorders (bacpacorders@chori.org).  Any scientific questions about available resources: please email bacpac (bacpac@chori.org).  Phone messages: 510-450-7917 and 510-450-7919.  Fax: 510-450-7924 and 510-450-7951.

 

© 2005 Children's Hospital Oakland Research Institute
5700 Martin Luther King Jr Way • Oakland, California 94609
Phone 510-450-7600 • Fax 510-450-7910
Site MapDisclaimer