Email: ssmith@chori.org
Phone: 510-450-7675
Fax: 510-450-7910

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SELECTED PUBLICATIONS

  1. Witkowski, A., Joshi, A.K., and Smith, S. (2007). Coupling of the de novo fatty acid biosynthesis and lipoylation pathways in mammalian mitochondria. J Biol Chem 282, 14178-14185.
  2. Smith, S., and Tsai, S.-C. (2007). The Type I fatty acid and polyketide synthases: A tale of two megasynthases. Nat. Prod. Rep. 24, 1041-1072.
  3. Pasta, S., Witkowski, A., Joshi, A.K., and Smith, S. (2007). Catalytic residues are shared between two pseudosubunits of the dehydratase domain of the animal fatty acid synthase. Chem. Biol. in press.
  4. Bunkoczi, G., Pasta, S., Joshi, A.K., Wu, X., Kavanagh, K.L., and Smith, S. (2007). Mechanism and substrate recognition of human holo ACP synthase. Chem. Biol. 14, in press.
  5. Smith, S. (2006). Structural biology. Architectural options for a fatty acid synthase. Science 311, 1251-1252.
  6. Zhang, L., Joshi, A.K., Hofmann, J., Schweizer, E., and Smith, S. (2005). Cloning, expression, and characterization of the human mitochondrial beta-ketoacyl synthase. Complementation of the yeast CEM1 knock-out strain. J Biol Chem 280, 12422-12429.
  7. Joshi, A.K., Witkowski, A., Berman, H.A., Zhang, L., and Smith, S. (2005). Effect of modification of the length and flexibility of the acyl carrier protein-thioesterase interdomain linker on functionality of the animal Fatty Acid synthase. Biochemistry 44, 4100-4107.
  8. Asturias, F.J., Chadick, J.Z., Cheung, I.K., Stark, H., Witkowski, A., Joshi, A.K., and Smith, S. (2005). Structure and molecular organization of mammalian fatty acid synthase. Nat Struct Mol Biol 12, 225-232.
  9. Witkowski, A., Joshi, A.K., and Smith, S. (2004). Characterization of the beta-carbon processing reactions of the mammalian cytosolic fatty acid synthase: role of the central core. Biochemistry 43, 10458-10466.
  10. Witkowski, A., Ghosal, A., Joshi, A.K., Witkowska, H.E., Asturias, F.J., and Smith, S. (2004). Head-to-head coiled arrangement of the subunits of the animal fatty acid synthase. Chem Biol 11, 1667-1676.
  11. Zhang, L., Joshi, A.K., and Smith, S. (2003). Cloning, expression, characterization and interaction of two components of a human mitochondrial fatty acid synthase: malonyl transferase and acyl carrier protein. J Biol Chem 278, 40067-40074.
  12. Smith, S., Witkowski, A., and Joshi, A.K. (2003). Structural and functional organization of the animal fatty acid synthase. Prog Lipid Res 42, 289-317.
  13. Joshi, A.K., Zhang, L., Rangan, V.S., and Smith, S. (2003). Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity. J Biol Chem 278, 33142-33149.
  14. Joshi, A.K., Rangan, V.S., Witkowski, A., and Smith, S. (2003). Engineering of an active animal Fatty Acid synthase dimer with only one competent subunit. Chem Biol 10, 169-173.
  15. Witkowski, A., Joshi, A.K., and Smith, S. (2002). Mechanism of the beta-ketoacyl synthase reaction catalyzed by the animal fatty acid synthase. Biochemistry 41, 10877-10887.
  16. Rangan, V.S., and Smith, S. (2002). Fatty Acid Synthesis in Eukaryotes. In Biochemistry of lipids, lipoproteins and membranes, D.E. Vance and J. Vance, eds. (Amsterdam: Elsevier).
  17. Rangan, V.S., Joshi, A.K., and Smith, S. (2001). Mapping the functional topology of the animal fatty acid synthase by mutant complementation in vitro. Biochemistry 40, 10792-10799.
  18. Li, J., Derewenda, U., Dauter, Z., Smith, S., and Derwenda, Z. (2000). Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef-binding protein. Nature Structural Biology 7, 555-559.
  19. Witkowski, A., Joshi, A.K., Rangan, V.S., Fallick, A.M., Witkowska, H.E., and Smith, S. (1999). Dibromopropanone Cross-Linking of the Phosphopantetheine and Active-Site Cysteine Thiols of the Animal Fatty Acid Synthase Can Occur Both Inter and Intra-Subunit: re-evaluation of the side-by-side, antiparallel subunit model. J. Biol. Chem. 274, 11557-11563.
  20. Witkowski, A., Joshi, A.K., Lindqvist, Y., and Smith, S. (1999). Conversion of a ß-ketoacyl synthase to a malonyl decarboxylase by replacement of the active-site cysteine with glutamine. Biochemistry 38, 11643-11650.
  21. Joshi, A.K., Witkowski, A., and Smith, S. (1998). The malonyl/acetyltransferase and b-ketoacyl synthase domains of the animal fatty acid synthase can cooperate with the acyl carrier protein domain of either subunit. Biochemistry 37, 2515-2523.
  22. Witkowski, A., Joshi, A.K., and Smith, S. (1997). Characterization of the interthiol acyltransferase reaction catalyzed by the b-ketoacyl synthase domain of the animal fatty acid synthase. Biochemistry 36, 16338-16344.
  23. Rangan, V.S., and Smith, S. (1997). Alteration of the substrate specificity of the malonyl-CoA/acetyl-CoA:ACP S-acyltransferase domain of the multifunctional fatty acid synthase by mutation of a single arginine residue. J. Biol. Chem. 272, 11975-11978.
  24. Rangan, V.S., Serre, L., Witkowska, H.E., Bari, A., and Smith, S. (1997). Characterization of the malonyl-/acetyl transferase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro. Protein Engineering 10, 561-566.
  25. Joshi, A.K., Witkowski, A., and Smith, S. (1997). Mapping of functional interactions between domains of the animal fatty acid synthase by mutant complementation in vitro. Biochemistry 36, 2316-2322.
  26. Witkowski, A., Joshi, A.K., and Smith, S. (1996). Fatty acid synthase: In vitro complementation of inactive mutants. Biochemistry 35, 10569-10575.
  27. Rangan, V.S., Oskouian, B., and Smith, S. (1996). Identification of an inverted CCAAT box motif in the fatty acid synthase gene as an essential element for mediation of transcriptional regulation by cAMP. J. Biol. Chem. 271, 2307-2312.
  28. Smith, S. (1994). The animal fatty acid synthase: One gene, one polypeptide, seven enzymes. FASEB J. 8, 1248-1259.
  29. Amy, C.M., Williams-Ahlf, B., Naggert, J., and Smith, S. (1992). Intron-exon organization of the gene for the multifunctional animal fatty acid synthase. Proc. Natl. Acad. Sci. USA 89, 1105-1108.
  30. Amy, C.M., Williams-Ahlf, B., Naggert, J., and Smith, S. (1990). Molecular cloning of the mammalian fatty acid synthase gene and identification of the promoter region. Biochem. J. 271, 675-679.
  31. Amy, C., Witkowski, A., Naggert, J., Williams, B., Randhawa, Z., and Smith, S. (1989). Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthetase. Proc. Natl. Acad. Sci. U.S.A. 86, 3114-3118.
  32. Randhawa, Z.I., and Smith, S. (1987). Complete amino acid sequence of the medium-chain S-acyl fatty acid synthetase thioester hydrolase from rat mammary gland. Biochemistry 26, 1365-1373.
  33. Stern, A., Sedgwick, B., and Smith, S. (1982). The free coenzyme A requirement of animal fatty acid synthetase. J. Biol. Chem. 257, 799-803.
  34. Sedgwick, B., and Smith, S. (1981). The coenzyme A requirement of mammalian fatty acid synthetase. Arch. Biochem. Biophys. 208, 365-379.
  35. Libertini, L.J., and Smith, S. (1979). Synthesis of long chain acyl-enzyme thioesters by modified fatty acid synthetases and their hydrolysis by a mammary gland thioesterase. Arch. Biochem. Biophys. 192, 47-60.
  36. Lin, C.Y., and Smith, S. (1978). Properties of the thioesterase component obtained by limited trypsinization of the fatty acid synthetase multienzyme complex. J. Biol. Chem. 253, 1954-1963.
  37. Libertini, L.J., and Smith, S. (1978). Purification and properties of a thioesterase from lactating rat mammary gland which modifies the product specificity of fatty acid synthetase. J. Biol. Chem. 253, 1393-1401.
  38. Smith, S., Agradi, E., Libertini, L., and Dileepan, K.N. (1976). Specific release of the thioesterase component of the fatty acid synthetase complex by limited trypsinization. Proc. Natl. Acad. Sci. USA 73, 1184-1188.
  39. Smith, S., and Abraham, S. (1975). Fatty acid synthase from lactating rat mammary gland. Meth. Enzymol. 35, 65-74.
  40. Smith, S., and Abraham, S. (1970). Fatty acid synthetase from lactating rat mammary gland.  I. Isolation and properties. J. Biol. Chem. 245, 3209-3217.
  41. Smith, S., and Dils, R. (1964). The factors affecting the chain-length of fatty acid synthesized by lactating rabbit mammary gland. Biochim. Biophys. Acta 84, 776-778.


Revised: Tuesday, January 24, 2012 11:49 AM

 

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