Recent Publications

ApoE structure and LDL receptor interactions

Hauser, P.S. and Ryan, R.O. (2007) Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein. Protein Expr. Purif.  In press.

Yamamoto, T. and Ryan, R.O. (2007) Anionic phospholipids inhibit apolipoprotein E – low-density lipoprotein receptor interactions.  Biochem. Biophys. Res. Commun. 354, 820-824.

Redmond, K.A., Murphy, C., Kiss, R.S., Hauser, P, Guigard, E., Kay, C.M. and Ryan, R.O. (2006) Replacement of helix 1' enhances the lipid binding activity of apoE3 N-terminal domain.  FEBS Journal, 273, 558-567.

Gupta, V., Narayanaswami, V., Budamagunta, M.S., Yamamoto, T., Voss, J.C. and Ryan, R.O. (2006) Lipid induced extension of apolipoprotein E helix 4 correlates with low density lipoprotein receptor binding ability.  J. Biol. Chem. 281, 39294-39299.

Yamamoto, T., Lamoureux, J. and Ryan, R.O. (2006) Characterization of low-density lipoprotein receptor ligand interactions by fluorescence resonance energy transfer.  J. Lipid Res. 47, 1091-1096.

Yamamoto, T. and Ryan, R.O. (2006) Role of leucine zipper motif in apoE3 N-terminal domain lipid binding activity.  Biochim. Biophys. Acta. 1761, 1100-1106.

Narayanaswami, V. Maiorano, J.N., Dhanasekaran, P. Ryan, R.O. Phillips, M.C., Lund-Katz, S. and Davidson, W.S. (2004) Helix Orientation of the Functional Domains in Apolipoprotein E in Discoidal High Density Lipoprotein Particles. J. Biol. Chem. 279, 14273-14279.

Weers, P.M.M., Narayanaswami, V., Choy, N., Luty, R., Hicks, L., Kay, C.M. and Ryan, R.O. (2003) Lipid binding ability of human apoE N-terminal domain isoforms: Correlation with protein stability? Biophysical Chemistry 100, 481-492.

Kiss R.S., Weers, P.M.M., Narayanaswami, V., Cohen, J., Kay, C.M. and Ryan, R.O. (2003) Structure guided protein engineering modulates helix bundle exchangeable apolipoprotein properties. J. Biol. Chem.  278, 21952-21959.

Raussens, V., Slupsky, C.M., Sykes, B.D. and Ryan, R.O. (2003) Lipid bound structure of an apolipoprotein E derived peptide. J. Biol. Chem. 278, 25998-26006.

Raussens, V., Slupsky, C.M., Ryan, R.O. and Sykes, B.D. (2002) NMR studies of a receptor-active apolipoprotein E peptide. J. Biol. Chem. 277, 29172-29180.

Apolipoprotein A-V and regulation of plasma triacylglycerol homeostasis

Nilsson, S.K., Lookene, A., Beckstead, J.A., Gliemann, G. Ryan, R.O. and Olivecrona, G. (2007) Apolipoprotein A-V interaction with members of the Low Density Lipoprotein Receptor Gene Family. Biochemistry, 46, 3896-3904.

Beckstead, J.A. Wong, K., Gupta, V., Wan, C.-P. L., Cook, V.R., Weinberg, R.B., Weers, P.M.M., and Ryan, R.O. (2007) The effect of C-terminal truncation on the structural and lipid binding properties of apolipoprotein A-V.  J. Biol. Chem. Papers in Press, March 31, 2007.

Wong, K. and Ryan, R.O. (2007) Characterization of apolipoprotein A-V structure and mode of plasma triacylglycerol regulation.  Current Opin. Lipidol. In Press.

Beigneux, A.P., Davies, B., Gin, P., Weinstein, M.M., Farber, E., Qiao, X., Peale, F., Bunting, S., Walzem, R.L., Wong, J.S., Blaner, W.S., Ding Z., Melford, K., Wongsiriroj, N., Shu, X., de Sauvage, F., Ryan, R.O., Fong, L.G., Bensadoun, A.  Young, S.G. (2007) Glycosylphosphatidylinositol-anchored high density lipoprotein–binding protein 1 plays a critical role in the lipolytic processing of chylomicrons. Cell Metabolism, 5, 279-291.

Lookene, A., Beckstead, J.A., Nilsson, S., Olivecrona, G. and Ryan, R.O. (2005) Apolipoprotein A-V heparin interactions.  Implications for plasma lipoprotein metabolism.  J. Biol. Chem. 280, 25383-25387.

Beckstead, J.A., Oda, M.N., Martin, D.D.O., Forte, T.M., Bielicki, J.K., Berger, T., Luty, R., Kay, C.M. and Ryan, R.O. (2003) Structure-function studies of human Apolipoprotein A-V: a regulator of plasma lipid homeostasis. Biochemistry, 42, 9416-9423.

Weinberg, R.B., Cook, V.R., Beckstead, J.A. Martin, D.D.O., Gallagher, J.W., Shelness, G.S. and Ryan, R.O. (2003) Structure and interfacial properties of human apolipoprotein A-V.  J. Biol. Chem. 278, 34438-34444.

Nanodisk drug delivery vehicle

Tufteland, M., Pesavento, J.B., Bermingham, R.L. Hoeprich, P.D. Jr. and Ryan, R.O. (2007) Peptide stabilized amphotericin B nanodisks.  Peptides, 28, 741-746.

Redmond, K.A., Nguyen, T.-S. and Ryan, R.O. (2007) All-trans retinoic acid nanodisks. Int. J. Pharm. In Press.

Oda, M.N., Hargreaves, P, Beckstead, J.A., Redmond, K.A., van Antwerpen, R. and Ryan, R.O. (2006) Reconstituted high-density lipoprotein enriched with the polyene antibiotic, amphotericin B.  J. Lipid Res.  47, 260-267.

Hargreaves, P.L., Nguyen, T-S. and Ryan, R.O. (2006) Spectroscopic studies of amphotericin B solubilized in nanoscale bilayer membranes.  Biochim. Biophys. Acta 1758, 38-44.

Nelson, K.G., Bishop, J., Ryan, R.O. and Titus, R. (2006) Nanodisk-associated amphotericin B clears Leishmania major cutaneous infection in susceptible BALB/c mice. Antimicrob. Agents Chemother.  50, 1238-1244.

Studes of other apolipoproteins

Martin, D.D.O., Budamagunta, M.S., Ryan, R.O., Voss, J.C. and Oda, M.N. (2006) Apolipoprotein A-I assumes a “looped belt” conformation on reconstituted high density lipoprotein. J. Biol. Chem. 281, 20418-20426.

Weers, P.M.M. and Ryan, R.O. (2006) Apolipophorin III: role model apolipoprotein.  Insect Biochem. Mol. Biol. 36, 231-240.

Beckstead, J.A., Block, B.L., Bielicki, J.K., Kay, C.M., Oda, M.N. and Ryan, R.O. (2005) Combined N- and C-terminal truncation of human apolipoprotein A-I yields a folded, functional central domain.  Biochemistry 44, 4591-4599.

Van der Horst, D.J. and Ryan, R.O. (2004) Lipid Transport. In: Comprehensive Insect Physiology, Biochemistry, Pharmacology and Molecular Biology (L.I. Gilbert, K. Iatrou and S. Gill, Eds) Elsevier, Oxford. Vol. 4, pp. 225-246.

Oda, M.N., Forte, T.M., Ryan, R.O. and Voss, J.C. (2003) The C-terminal domain of apolipoprotein A-I contains a lipid sensitive conformational trigger.  Nature Structural Biology 10, 455-460.

Ryan, R.O., Forte T.M. and Oda, M.N. (2003) Optimized bacterial expression of human apolipoprotein A-I. Protein Expr. Purif. 27, 98-103.

Weers, P.M.M. and Ryan, R.O. (2003) Apolipophorin III: a lipid-triggered molecular switch.  Insect Biochem. Mol. Biol.  33, 1249-1260.

Sahoo, D., Weers, P.M.M., Ryan, R.O. and Narayanaswami, V. (2002) Lipid triggered molecular switch of apoLp-III helix bundle to an extended helix conformation. J. Mol. Biol. 321, 201-214.